《植物生理学报》 2010, 46(1): 1-4

巴西橡胶树的脂酰辅酶 A 还原酶 cDNA 克隆及其序列分析

罗明武¹, 邓柳红^2,*

1 海南大学材料与化工学院, 海口 570228; 2 中国热带农业科学院热带生物技术研究所, 海口 571101

通信作者：邓柳红；E-mail: dengliuhong168@163.com；Tel: 0898-66892946

摘　要：

从巴西橡胶树差减cDNA文库中筛选到一个与脂酰辅酶A还原酶同源性较高的基因片段, 根据该基因片段序列信息, 设计特异引物, 采用 RACE 进行差异片段的 5' 和 3' 端的扩增, 获得长度为 1 365 bp 的 cDNA 克隆 R28 (GenBank 登陆号: AY461413)。序列分析表明, 该基因包含 1 149 bp 的开放阅读框, 5'-UTR 为 96 bp, 3'-UTR 为 128 bp, 编码 382 个氨基酸, 推测 其蛋白质的分子量为43.5 kDa, 等电点为8.97, 有一个跨膜螺旋区(187 至215位氨基酸)和1 个由17个氨基酸组成的信号肽(1 至17 位氨基酸)。R28含有脂酰辅酶A还原酶的保守区(NADP结合蛋白保守区), 推测该基因是一个脂酰辅酶A还原酶基因。

关键词：巴西橡胶树; 胶乳; 酰基辅酶A还原酶

收稿：2009-08-31   修定：2009-12-09

资助：中国热带农业科学院科技基金和华南热带农业大学科技基金。

Cloning and Sequence Analysis of Acyl-CoA Reductase cDNA from Hevea brasiliensis Muell. Arg.

LUO Ming-Wu¹, DENG Liu-Hong^2,*

¹College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China;²Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Corresponding author: DENG Liu-Hong; E-mail: dengliuhong168@163.com; Tel: 0898-66892946

Abstract:

A cDNA clone related to lipid transportation and metabolism was isolated by screening subtracted latex cDNA library. According to its sequences information, a novel full-length cDNA termed R28 (GenBank number: AY461413) was obtained by using rapid amplification of cDNA ends (RACE). R28 was 1 365 bp long containing a 1 149-bp ORF, flanked by a 96-bp 5'-UTR and a 128-bp 3'-UTR. It encoded 382 amino acids with a theoretical molecular weight of 43.5 kDa and an isoelectric point of 8.97. Hydropathy and transmembrane motif analysis of deduced amino acid sequences indicated that R28 possessed a transmembrane spanning domain (185−215 aa), and contained a cleaved signal peptide (1−17 aa). The result of the conserved domains analysis indicated that R28 not only has a highly conserved region of acyl-CoA reductase, but also contains a male sterility protein C-terminal region. Multialignment revealed that R28 is the most closely related to the jojoba acyl CoA reductase.

Key words: Hevea brasiliensis; latex; acyl-CoA reductase